Burden of Arthropod-borne viruses (Arboviruses) increasing constantly in recent years causing health problem worldwide. Also in Europe these viruses were increasingly reported, with relevant health problems, like the Chikungunya epidemic in Italy in 2007, with about 200 human cases.
The surveillance of those viruses could be achieved by surveillance on arthropod vectors. Entomological surveillance can highlight the circulation of a virus early in the season and providing quantifiable information on intensity of virus transmission by data on mosquito infection rates, and consequently providing information on potential risk to humans and animals. Field data confirmed that, if mosquito trapping effort is intensive, detection of Arbovirus in mosquitoes precede appearance of animal and human cases.
IZSLER collaborate to different arbovirus surveillance plans, particularly based on mosquito monitoring. In these plans arthropods are sampled (e.g. by attractive traps), grouped (according to species, place and day of sampling), ground and submitted to isolation on cell culture and biomolecular analysis for detection of searched viruses.
The Biobank gives the opportunity to conserve sampled arthropods after analysis for future research works. Particularly these samples could be useful to study the population genetic of collected mosquitoes, or detect further viruses and discovering new ones possibly present in the samples. Moreover the Biobank will form over the years an historical archive over the years, able to give feedback to the presence of pathogens on the surveyed territory.
Sampled arthropods are identified according to adequate morphological keys and place, day and number of specimens per pool recorded. Applied PCRs on collected pools are recorded.
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BIOBANK infrastructure of IZSLER has the mission of collecting all biological resources submitted to purity and identity controls, stored in different laboratories. It is difficult to get from others biological samples with known characteristics except for those that are obtained from certified Centres.
To cover this gap all biological resources collected at IZSLER biobank have been controlled and characterised by standard protocols to have detailed information on their main features. These data are included in a data base published in this web site.
Biobank infrastructure is made from best of breed technology to preserve all biological resources stored by IZSLER laboratories.
Maria Beatrice Boniotti (Biobank Manager)
Phone: +39 (030) 2290-263 / 273
Fax: +39 (030) 2290-386