The cell culture collection of
IZSLER Biobank includes over 600 cell types subdivided into the following five
categories: established cell lines (CL), tumor cell lines (TCL), primary cell
cultures (PRC), mesenchymal stromal cells (MSC) and hybridomas (HY).
The established, tumor and
primary cell cultures have been isolated from different tissues and organs of
several different species.
On the contrary, mesenchymal
stromal cells have been isolated only from animals, mainly of equine, feline
and canine species and obtained either from bone marrow or adipose tissue.
All cell types are amplified,
controlled in their proper characteristics and quality parameters and
cryopreserved in nitrogen vapor tanks.
All cell types are submitted
to quality control tests in order to evaluate their characteristics, purity and
identity. These controls are carried out by different techniques in the field
of cell biology, by virology and microbiologic testing, throughout molecular
biology reactions and by in vivo investigations.
Tests are carried out
according to international guidelines such as those indicated by the European
Detection of oncogenic
features of cell cultures can be detected by in vivo tests that are carried out
by inoculation of cells into laboratory animals as indicated in the European
Pharmacopeia. Tumor formation is evaluated in the inoculated animals and
confirmed by histology assay.
Moreover, the ability of cells
to form colonies is detected by inoculation in an appropriate semi-solid
The highest risk that can
occur in in cell culture laboratories is contamination by microorganisms and
viruses that can be present in the tissue / organ of the donors from which
cells have derived, from animal reagents or that can result from improper
Mycoplasma can be detected by
the below indicated techniques:
A broad range of viruses can
contaminate cell cultures and viral detection can be performed by the below
Another major risk in cell
repositories is represented by the misidentified cell lines due to the
circulation of false cells that are currently used in laboratories. Their
identification can be confirmed by the following reported techniques:
Quality management system
The Cell Culture Laboratory
perform in according to ISO EN IEC 17025 quality system and achieved ISO 9001
certification for its activity.
Prices and time for
Click here to browse Cell Cultures Biobank
Download Cell Cultures Biobank catalog