The cell culture collection of IZSLER Biobank includes over 600 cell types subdivided into the following five categories: established cell lines (CL), tumor cell lines (TCL), primary cell cultures (PRC), mesenchymal stromal cells (MSC) and hybridomas (HY). Furthermore, tissues and samples from patients affected by Alzheimer Disease (ALZ) are stored as well.
The established, tumor and primary cell cultures have been isolated from different tissues and organs of several different species.
On the contrary, mesenchymal stromal cells have been isolated only from animals, mainly of equine, feline and canine species and obtained either from bone marrow or adipose tissue.
All cell types are amplified, controlled in their proper characteristics and quality parameters and cryopreserved in nitrogen vapor tanks.
All cell types are submitted to quality control tests in order to evaluate their characteristics, purity and identity. These controls are carried out by different techniques in the field of cell biology, by virology and microbiologic testing, throughout molecular biology reactions and by in vivo investigations.
Tests are carried out according to international guidelines such as those indicated by the European Pharmacopeia.
Detection of oncogenic features of cell cultures can be detected by in vivo tests that are carried out by inoculation of cells into laboratory animals as indicated in the European Pharmacopeia. Tumor formation is evaluated in the inoculated animals and confirmed by histology assay.
Moreover the ability of cells to form colonies is detected by inoculation in an appropriate semi-solid medium.
The highest risk that can occur in in cell culture laboratories is contamination by microorganisms and viruses that can be present in the tissue / organ of the donors from which cells have derived, from animal reagents or that can result from improper laboratory practice.
Mycoplasma can be detected by the below indicated techniques:
A broad range of viruses can contaminate cell cultures and viral detection can be performed by the below indicated techniques:
Another major risk in cell repositories is represented by the misidentified cell lines due to the circulation of false cells that are currently used in laboratories. Their identification can be confirmed by the following reported techniques:
Quality management system
The Cell Culture Laboratory perform in according to ISO EN IEC 17025 quality system and achieved ISO 9001 certification for its activity.
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