The hybridoma collection of IZSLER Biobank contains an initial selection of those hybridoma that produce monoclonal antibodies regularly used in in-house diagnostic assays. They were generated within IZSLER Biotechnology Laboratory, and were raised towards viruses of veterinary interest and immunoglobulin isotypes of various animal species.
The hybridoma currently included in the Biobank are a sample of the many hybridoma generated during 30 years of research and development activities conducted at IZSLER to produce monoclonal antibodies against a wide spectrum of viruses, bacteria and proteins, mainly of veterinary interest, that were shown to be strategic tools for both research and diagnostic purposes. Of these hybridoma, the most relevant have been also identified for being gradually transferred into the IZSLER Biobank after submission to further quality controls, including a second cloning process.
Quality controls
Viability
Hybridoma cultures are usually collected for freezing during the exponential growth phase; the number of cells per vial is known.
Purity
Hybridoma are submitted to a double series of cloning procedure to assure the stability of the hybridoma and the clonality of the antibody produced.
Authenticity
The monoclonal antibodies expressed by each hybridoma are controlled through a series of immunological assays aimed at identifying and characterising their reactivity profile.
Quality management system
IZSLER laboratories, that supply biological resources to IBVR, use testing methods performed according to UNI EN ISO/IEC 17025 quality system.
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BIOBANK infrastructure of IZSLER has the mission of collecting all biological resources submitted to purity and identity controls, stored in different laboratories. It is difficult to get from others biological samples with known characteristics except for those that are obtained from certified Centres. To cover this gap all biological resources collected at IZSLER biobank have been controlled and characterised by standard protocols to have detailed information on their main features. These data are included in a data base published in this web site. Biobank infrastructure is made from best of breed technology to preserve all biological resources stored by IZSLER laboratories.