Hybridomas

The hybridoma collection of IZSLER Biobank contains an initial selection of those hybridoma that produce monoclonal antibodies regularly used in in-house diagnostic assays. They were generated within IZSLER Biotechnology Laboratory, and were raised towards viruses of veterinary interest and immunoglobulin isotypes of various animal species.

The hybridoma currently included in the Biobank are a sample of the many hybridoma generated during 30 years of research and development activities conducted at IZSLER to produce monoclonal antibodies against a wide spectrum of viruses, bacteria and proteins, mainly of veterinary interest, that were shown to be strategic tools for both research and diagnostic purposes. Of these hybridoma, the most relevant have been also identified for being gradually transferred into the IZSLER Biobank after submission to further quality controls, including a second cloning process.

Quality controls

Viability

Hybridoma cultures are usually collected for freezing during the exponential growth phase; the number of cells per vial is known.

Purity

Hybridoma are submitted to a double series of cloning procedure to assure the stability of the hybridoma and the clonality of the antibody produced.

Authenticity

The monoclonal antibodies expressed by each hybridoma are controlled through a series of immunological assays aimed at identifying and characterising their reactivity profile.

Quality management system

IZSLER laboratories, that supply biological resources to IBVR, use testing methods performed according to UNI EN ISO/IEC 17025 quality system.

 

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