The order Mycoplasma includes
many pathogens of humans and animals.
The majority of the strains
stored in the biobank are of veterinary interest and are causative agents of
The biobank contains reference
strains, that are selected among the pathogens of interest, and field strains
that are isolated from different species of animals. These strains are chosen
for the pathogenicity, the clinical signs caused in the animals and any other
relevant peculiarity, that are explained in each strains card.
Moreover, also the DNA of some
fastidious strains that are difficult to grow and are important in veterinary
pathology can be found in the Mycoplasma strains collection.
The reference strains are
cultured to evaluate their viability before the storage.
Once isolated from the field
sample, the strain is classified for its morphology and growth behaviour and
purified by cloning. Than it is identified by the most significant techniques
available for this purpose (OIE terrestrial manual, publications) and by our
standardized methods. Biochemical and/or molecular tests, including sequencing,
are adopted; the identification of the strains in some case can be confirmed by
other reference laboratories.
Molecular assays can be
performed directly on the tissue or diagnostic sample, if the culture fails and
a Mycoplasma infection is suspected; the identification of the strains is done
by sequencing the PCR product.
From all strains provided for
deposit, viability and purity are tested by subculturing, after intervals
scheduled for the different strains.
Quality management system
IZSLER laboratories, that
supply biological resources to IBVR, use testing methods performed according to
UNI EN ISO/IEC 17025 quality system.
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