Chlamydiaceae

Microorganisms belonging to the Chlamydiaceae family are Gram-negative, obligatory intracellular bacteria involved in diseases affecting both animals and humans.

The Chlamaydiaceae family traditionally contained a single genus, Chlamydia (Bergey’s Manual of Systematic Bacteriology vol.1, 1984) but in 1999 another genus, Chlamydophila was added based on 16S rRNA and 23S rRNA sequence’s similarity (Everett et al. in 1999). Furthermore, other five new species were proposed by Everett. However, the use of the new genus name is still debated within the scientific community and for this reason the new edition of the Bergey’s Manual (vol.4, 2011) considers the genus Chlamydia the only genus and the five additional species are classified within this genus. Since the taxonomy of the family Chlamydiaceae is currently under consideration, and species with the name Chlamydophila felis, Chlamydophila pecorum and Chlamydophila abortus have been widely and validly published, the subdivision into two genera Chlamydia and Chlamydophila has been retained in this collection until 2013. From 2014, only the genus Chlamydia will be used.

The collection consists of field strains isolated from animals and includes C.psittaci (from birds and mammals), C. abortus, C. pecorum, C. felis, and C. suis.

Quality controls

All strains enrolled in our collection are submitted to different molecular biology control tests in order to evaluate their viability, purity and identity:

Viability

It is performed on cell cultures before freezing and after thawing in order to evaluate the cryopreservation efficiency. The growth of the strains is evaluated by immunofluorescence test (IFT).

Purity and Identity

All isolates were tested by different molecular biology techniques, such as: specie-specific real-time PCR and PCR RFLP targeting the 16S and 23S rRNA genes and ompA gene that allow to confirm the purity and the identity of each isolate.

Quality management system

IZSLER laboratories, that supply biological resources to IBVR, use testing methods performed according to UNI EN ISO/IEC 17025 quality system.

 

Click here to browse Chlamydiaceae Biobank

 


Click here to download Chlamydiaceae Biobank catalog