achlorophyllous yeast-like microalgae belonging to the genus Prototheca
(Trebouxiophyaceae, Chlorophyta) were first described by Kruger in 1894.
Basically, the genus Prototheca encompasses six species: P. cutis, P. stagnora,
P. ulmea, P. wickerhamii, P. zopfii and P. blaschkeae. (Pore. 1985; Roesler et
al. 2006). A seventh species, P. moriformis, is not currently accepted because
of its biochemical/genetic resemblance with P. zopfii, (Roesler et al. 2006),
which was differentiated into two genotypes: genotype 1 and genotype 2 on the
basis of biochemical, serological and 18S-rDNA sequencing data. (Roesler et al.
2006). These microalgae are ubiquitous inhabitants in the environment and
contaminants of many substrates. (Nelson et al. 1987). P. zopfii, P.
blaschkeae, P. wickerhamii and P. cutis may determine persisting infections in
the presence of impaired immunological-cellular systems and/or poor animal care
(Lass-Flörl et al. 2007; Jánosi et al. 2001) in both humans and animals.
Our collection encompasses some well characterised
field isolates and other strains coming from other cellular banks.
enrolled in our collection are submitted to different cultural and molecular
biology control tests in order to evaluate their characteristics, purity and
were screened for the growth in specific prototheca medium (PIM). Then, a
single colony was sub-cultured on Sabouraud Agar and growth at 30°C for 48-72h.
performed on all cell cultures prior to freezing and after thawing in order to
evaluate the cryopreservation efficiency. Cells were plated on Sabouraud Agar
and grown at 30°C for 48-72h.
of each isolate has been confirmed through different molecular biology
techniques, such as: direct sequencing of rRNA 18S, species/genotype specific
PCR3 and PCR/DNA resolution melting analysis. Ricchi et al. 2011).
laboratories, that supply biological resources to IBVR, use testing methods
performed according to UNI EN ISO/IEC 17025 quality system.
Updated to July 2022
Click here to browse Prototheca Algae Biobank
Click here to download Prototheca algae Biobank catalog